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It depends on how the board is wired but from the looks of it one takes PCIe lanes from the CPU and the other uses SATA so it will disable some SATA ports and use that.

Even if your GPU runs at x8 mode it won't impact performance so you're good either way.

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For a B250 motherboard. All 16 lanes from your CPU is used up by the 1st x16 slot. Rest of the board that needs pcie lanes, takes it from the chipset. Intel B250 has 12 pcie lanes. This gives you a combined total of 28 pcie lanes.

 

 

 

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41 minutes ago, abdo601 said:

http://prntscr.com/fw31y7
 

more people told me i cant install only one m.2 on a B250 board

but why asus B250 has tow M.2 ports? https://prnt.sc/fw0ru8

According to the specsheet, one m2 port is connected to 4 PCIe 3 lanes while the second port is SATA only.

 

Source:

https://www.asus.com/Motherboards/ROG-STRIX-B250F-GAMING/specifications/

Desktop: Intel i9-10850K (R9 3900X died 😢 )| MSI Z490 Tomahawk | RTX 2080 (borrowed from work) - MSI GTX 1080 | 64GB 3600MHz CL16 memory | Corsair H100i (NF-F12 fans) | Samsung 970 EVO 512GB | Intel 665p 2TB | Samsung 830 256GB| 3TB HDD | Corsair 450D | Corsair RM550x | MG279Q

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