A weird pcie question.
You have 24 pci-e lanes coming from cpu : 4 go to first m.2 , 16 go to video cards (either one x16 or two slots x8, depends on motherboard maker) and 4 go to chipset.
The chipset creates 8 extra pci-e lanes, which are typically arranged in a second m.2 connector (2 or 4 pci-e lanes) and/OR a pci-e x16 slot that has only 4 pci-e lanes in it, and some pci-e x1 slots
Your board has 3 pci-e x16 slots ... the first two come from the cpu and use those 16 pci-e lanes from cpu. If you have a single video card in first slot, it gets all 16 pci-e lanes. If you plug something in that second pci-e slot, both slots become pci-e x8. Will you feel a performance loss in some games? Yeah probably, but it's gonna be in the 1-2% maximum , so a few fps at best.
The bottom pci-e x16 slot has 4 pci-e lanes which come from the chipset.
It shares bandwidth with the pci-e x1 slot right above it, so you probably wouldn't want a sound card installed in that slot, if you install the capture card in the bottom pci-e slot (because it may affect the transfer speeds in the capture card, if it captures raw video) but it's worth testing ... sound cards don't use a lot of bandwidth.
The M.2 connector will stay at pci-e 4.0 speed if the ssd supports pci-e 4.0
The two pci-e x16 slots will both switch to pci-e 3.0 if your video card only supports pci-e 3.0

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