I can usually determine the frame rate of a game up to about 40 frames per second by just looking and moving around. Past about 40, I have a +/- 4 FPS range to about 60.
And I can tell when frame rates are above 60 FPS because then I get terrible image tearing in games on my 60 Hz monitor :P
​But, to address the OP, yes. There's quite a noticeable difference.