Can I boot from an M.2 through chipset lanes?

[Flux Azreal]

So I built a system using the MSI B550-A PRO motherboard and it has 2 m.2 lanes, 1 is through the cpu (4.0 x4) and 1 through mb chipset (3.0 x4) and I wanted to get another m.2, though I was wondering if I can even boot from the chipset one, i'm not sure if its a universal thing or if it differs from mb to mb, so I was hoping if someone could help me find this info? My CPU is the Ryzen 5 5600 and both drives are nvme

[Electronics Wizardy]

Both of the m.2 slots are bootable.

[Agall]

40 minutes ago, Flux Azreal said:

So I built a system using the MSI B550-A PRO motherboard and it has 2 m.2 lanes, 1 is through the cpu (4.0 x4) and 1 through mb chipset (3.0 x4) and I wanted to get another m.2, though I was wondering if I can even boot from the chipset one, i'm not sure if its a universal thing or if it differs from mb to mb, so I was hoping if someone could help me find this info? My CPU is the Ryzen 5 5600 and both drives are nvme

In general, its quite flexible now. Any m.2 slot including m.2 interfaces uses PCIe expansion should be bootable in win10/11, whether AHCI or NVMe m.2 drives.